| Primer | Species | LP | RP | Tml | Tmr | Length | Score | Target | Senstive |
|---|---|---|---|---|---|---|---|---|---|
| Primer 1 | Anoxybacillus flavithermus | ACACACATCGTCGAGGAGC | ACTTACGACACCGCACTGG | 59.79 | 60.01 | 156 | 146 |
100.00%
|
100.00%
|
| Primer 2 | Anoxybacillus flavithermus | AAAGCGCTAGAGAGGGTGC | TGCAAACCCAGGCGATGT | 59.78 | 59.88 | 159 | 138 |
100.00%
|
100.00%
|
| Primer 3 | Anoxybacillus flavithermus | AGCATCCTGGAGCAGTTGC | TGCAAACCCAGGCGATGT | 60.38 | 59.88 | 230 | 138 |
100.00%
|
100.00%
|
| Primer 4 | Anoxybacillus flavithermus | AATGGCGTTCGTGGAGAGG | ATCGCTGTTGCCACATGC | 60.08 | 59.12 | 210 | 138 |
100.00%
|
100.00%
|
| Primer 5 | Anoxybacillus flavithermus | AATCGGTCCAACGGCTGT | GCCCTGTACCACCAACGAT | 59.25 | 59.70 | 284 | 131 |
100.00%
|
100.00%
|
| Primer 6 | Anoxybacillus flavithermus | TACAAGCGCTTGCGCAAC | GCCAGCCTCCTCTTGTTGT | 59.75 | 59.93 | 191 | 128 |
100.00%
|
100.00%
|
| Primer 7 | Anoxybacillus flavithermus | ACGGTTTGGACGATGGCA | GGACAGCTCATTCGCCACT | 59.57 | 60.08 | 189 | 128 |
100.00%
|
100.00%
|
| Primer 8 | Anoxybacillus flavithermus | ACGGTTTGGACGATGGCA | AGGACAGCTCATTCGCCAC | 59.57 | 60.08 | 190 | 128 |
100.00%
|
100.00%
|
| Primer 9 | Anoxybacillus flavithermus | AATCGGTCCAACGGCTGT | GTGAACGAGCAAGCGTCTG | 59.25 | 59.51 | 227 | 128 |
100.00%
|
100.00%
|
| Primer 10 | Anoxybacillus flavithermus | AATCGGTCCAACGGCTGT | TGAACGAGCAAGCGTCTG | 59.25 | 58.06 | 226 | 128 |
100.00%
|
100.00%
|